For staining Bacillus tuberculosis the following is confidently commended as preferable to the materials and methods heretofore in use. Take glycerine, 20 parts; fuchsin, 3 parts; aniline oil, 2 parts; carbolic acid, 2 parts.
The solution is readily and speedily effected, with no danger of precipitation, and can be kept in stock without risk of deterioration. When wanted for use, put about two drops into a watch glass (a small pomatum pot is better) full of water and gently shake or stir. Just here there is some danger of precipitating the coloring matter, but the difficulty is easily avoided by gentle instead of vigorous stirring. After the stain is once dissolved in the water no further trouble occurs; if any evaporation takes place by being left too long, it is the water that goes, not the main solvent. The color should now be a light, translucent red, much too diffuse for writing ink. Put in the smeared cover glass, after passing it a few times through a flame, and leave it, at the ordinary temperature of a comfortable room, half an hour.

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MPAA: G
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